Nanostructured biosensor for measuring neuropathy target esterase activity.
نویسندگان
چکیده
Neuropathy target esterase (NTE) is a membrane protein found in human neurons and other cells, including lymphocytes. Binding of certain organophosphorus (OP) compounds to NTE is believed to cause OP-induced delayed neuropathy (OPIDN), a type of paralysis for which there is no effective treatment. Mutations in NTE have also been linked with serious neurological diseases, such as motor neuron disease. This paper describes development of the first nanostructured biosensor interface containing a catalytically active fragment of NTE known as NEST. The biosensor was fabricated using the layer-by-layer assembly approach, by immobilizing a layer of NEST on top of multilayers consisting of a polyelectrolyte (poly-L-lysine) and an enzyme (tyrosinase). The biosensor has a response time on the order of seconds and gives a concentration-dependent decrease in sensor output in response to a known NEST (and NTE) inhibitor. Potential applications of the biosensor include screening OP compounds for NTE inhibition and investigating the enzymology of wild-type and mutant forms of NTE. Although the development of a NEST biosensor was the primary purpose of this study, we found that the approach developed for NEST could also be extended to measure the activity of other esterases involved in neural processes, such as acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). On the basis of measured sensitivities, phenyl valerate was the preferred substrate for NEST and BChE, whereas phenyl acetate was better for AChE.
منابع مشابه
Effect of vitamins B1, B6 and B12 (Neurobion) on diisopropylfluorophosphate–induced delayed neuropathy in mice
Certain organophosphorus esters such as diisopropylfluorophosphate (DFP) cause delayed neuropathy by inhibition of neuropathy target esterase (NTE) keeping neuron in normal function. In this study, effects of neurobion alone and in combination with dexamethasone on DFP–induced delayed neuropathy were evaluated. Thirty-five mice were divided to five groups that each group consists 7 mice. Except...
متن کاملEffect of vitamins B1, B6 and B12 (Neurobion) on diisopropylfluorophosphate–induced delayed neuropathy in mice
Certain organophosphorus esters such as diisopropylfluorophosphate (DFP) cause delayed neuropathy by inhibition of neuropathy target esterase (NTE) keeping neuron in normal function. In this study, effects of neurobion alone and in combination with dexamethasone on DFP–induced delayed neuropathy were evaluated. Thirty-five mice were divided to five groups that each group consists 7 mice. Except...
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Neuropathy target esterase (NTE) is the target protein for neuropathic organophosphorus (OP) compounds that produce OP compound-induced delayed neurotoxicity (OPIDN). Inhibition/aging of brain NTE within hours of exposure predicts the potential for development of OPIDN in susceptible animal models. Lymphocyte NTE has also found limited use as a biomarker of human exposure to neuropathic OP comp...
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Neuropathy target esterase (NTE) is a membrane-bound carboxylesterase activity that has been proposed as the target site for initiation of organophosphate-induced delayed neuropathy. This activity is identified by its resistance to treatment with Paraoxon and sensitivity to co-incubation with Paraoxon and Mipafox. Sucrose-density-gradient centrifugation of membrane-associated proteins isolated ...
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عنوان ژورنال:
- Analytical chemistry
دوره 79 14 شماره
صفحات -
تاریخ انتشار 2007